Fig. 2: The transcriptome of ASXL1^MT CMML is characterized by transcriptional up-regulation of key mitotic pathways and leukemogenic driver genes.

a Volcano plot showing a predominance of transcriptional up-regulation in ASXL1^MT CMML with a limited number of genes being down-regulated. Up-regulated therapeutic targets with therapeutic agents either being available or currently under development are labeled. Also labeled are the members of the posterior HOXA cluster including the leukemogenic driver HOXA9 and its co-factor MEIS1 (bold). b Heatmap showing the separation of ASXL1^MT and ASXL1^WT CMML by unsupervised hierarchical clustering of all differentially expressed genes with FDR < 0.010. c Circos plot showing the up-regulation of mitotic activity and down-regulation of MHC class I mediated antigen presentation and cytotoxic T-cell activity in ASXL1^MT CMML (red: up-regulated in ASXL^MT CMML, blue: up-regulated in ASXL^WT CMML). Bar graph showing the top hits in each gene ontology category (GO: Gene Ontology; BP: Biological Process; MF: Molecular Function; CC: Cellular Compartment). Axes represent the statistical significance of the gene ontology terms and the size of the markers is proportional to the number of genes per cluster.