Fig. 3: S100a9 gene is regulated by both Brca1 and S100a9. | Nature Communications

Fig. 3: S100a9 gene is regulated by both Brca1 and S100a9.

From: S100A9-CXCL12 activation in BRCA1-mutant breast cancer promotes an immunosuppressive microenvironment associated with resistance to immunotherapy

Fig. 3

a Expression of S100a9 and S100a8 in Brca1 MT (G600) and WT (B477) mammary epithelial cell lines. b Expression of S100a9 and S100a8 in B477 cells with the expression of shBrca1 at different concentrations. c Expression of S100a9 and S100a8 in B477 cells in which the mBrca1 gene was overexpressed. d Expression of S100A9 and S100A8 in MDA-MB-231 (231) control and 231 cells with the expression of shBRCA1(knock down BRCA1). e Luciferase activity assay of mouse S100a9 promoter after 72 h transfection with PGL vector only, S100a9 promotor, S100a9 promotor with Brca1 cDNA, and S100a9 promotor with S100a9 cDNA in B477 and G600 cells. f S100A9 proteins in B477 mouse mammary epithelial cells and 231 cells with the expression of shBrca1 or shBRCA1, respectively by IF. g BRCA1 and S100A9 protein levels in B477 mouse WT mammary epithelial cells and 231 cells without or with the expression of shBRCA1(shBr/shBR). The data are expressed as means ± SD (a–e) and P values determined by unpaired two-tailed Student’s t test (a, c, d) and by one-way ANOVA followed by Tukey’s multiple comparisons (b) or two-way ANOVA (e). The experiments were independently repeated three times with similar results (a–g). Scale bar: white color, 20 μM. Source data are provided as a Source data file.

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