Fig. 3: Different gene expression profiles between S100P + SPP1− and S100P-SPP1+ cells. | Nature Communications

Fig. 3: Different gene expression profiles between S100P + SPP1− and S100P-SPP1+ cells.

From: Single-cell transcriptomic analysis suggests two molecularly distinct subtypes of intrahepatic cholangiocarcinoma

Fig. 3

a Boxplot of the genomic heterogeneity (left) and transcriptomic heterogeneity (right) of S100P + SPP1−(n = 7) and S100P-SPP1 + (n = 7) iCCAs. (**P < 0.01; two-sided Wilcoxon rank-sum test; Transcriptomic heterogeneity: P = 0.0041; NS not significant). The central mark indicates the median, and the bottom and top edges of the box indicate the first and third quartiles, respectively. The top and bottom whiskers extend the boxes to a maximum of 1.5 times the interquartile range. b Top enriched pathways for genes with specific expression in S100P + SPP1− and S100P-SPP1 + cells. c Network representation of selected differentially expressed transcription factors between S100P + SPP1− and S100P-SPP1 + cells, as analyzed by SCENIC. Transcription factors in S100P + SPP1− are shown in red; transcription factors in S100P-SPP1 + are shown in blue. Bar graph showing the difference score for the selected set of differentially expressed transcription factors in S100P + SPP1− (red) and S100P-SPP1 + (blue). d Scatterplot showing the correlation of CREB3L1 expression (x-axis) with S100P expression (y-axis). Correlation is evaluated by the Spearman correlation coefficient. e The relative luciferase activity in HEK-293T cells following co-transfection with plasmid containing S100P promoter and increasing doses of the CREB3L1 expression vector (***P < 0.001; two-sided student’s t-test; CREB3L1 50 ng: P < 0.0001; 100 ng: P < 0.0001; 200 ng: P < 0.0001; n = 12 biologically independent samples). f, g Representative images of the Transwell invasion assay (f) and a statistical histogram (g) (***P < 0.001; two-sided student’s t-test; HuCCT1: P < 0.0001; RBE: P < 0.0001; n = 6 biologically independent samples). Scale bar, 100 μm. h Heatmap displaying expression levels of differentially expressed genes in Si-CREB3L1 versus Si-Ctl in HuCCT1 cells. i Top enriched pathways for downregulated genes in Si-CREB3L1 HuCCT1 cells. Si-Ctl Small interfering control (f, g, and h); NES normalized enrichment score (i). Error bars of (e and g) represent the means ± SD. Source data are provided as a Source Data file.

Back to article page