Fig. 4: Two different subsets of macrophages infiltrated in iCCAphl and iCCApps.
a The t-SNE plot showing the subtypes of myeloid cells derived from iCCA peri-tumor and tumor. b Heatmap showing the expression of marker genes in each subtype of myeloid cells. c t-SNE plot of myeloid cells from S100P + SPP1− (red dots) and S100P-SPP1 + (blue dots). d Bar plot showing the proportion of macrophage subsets from S100P + SPP1− and S100P-SPP1+. e, f Scatterplots showing pro-/anti-inflammatory scores (e) and M1/M2 scores (f) for two macrophage subsets. Macro_c1_SPP1, n = 4016 cells; Macro_c2_CCL18, n = 3447 cells. (***P < 0.001; two-sided Wilcoxon rank-sum test; Anti-inflammatory score: P < 2.22e-16; Pro-inflammatory score: P < 2.22e-16; M2 polarization score: P < 2.22e-16; M1 polarization score: P < 2.22e-16). The central mark indicates the median, and the bottom and top edges of the box indicate the first and third quartiles, respectively. The top and bottom whiskers extend the boxes to a maximum of 1.5 times the interquartile range. g, h Representative mIHC images (left) and statistical graphs (right) to show the distribution of CD68+SPP1+CCL18− and CD68+SPP1-CCL18+ macrophages in S100P+SPP1− (g) and S100P−SPP1 + (h), respectively: CK19 (green), S100P (red), SPP1 (purple), CD68 (white), CCL18 (yellow), and DAPI (blue) (S100P + SPP1− n = 68, S100P-SPP1 + n = 112). White arrows (CD68 + SPP1 + CCL18−), yellow arrows (CD68 + SPP1−CCL18+). (***P < 0.001; two-sided Mann–Whitney U-test; CD68 + SPP1 + CCL18− (%): P < 0.0001; CD68 + SPP1-CCL18 + (%): P < 0.0001). Data were presented as median with interquartile range (g and h). Scale bar, 50 μm. Source data are provided as a Source Data file.
