Fig. 6: p57⁺ cells acquire a stem cell-like transcriptomic profile in association with spatiotemporal reprogramming.

a CytoTRACE analysis of our scRNA-seq data. The higher the score, the greater the stem cell potential. The calculated CytoTRACE scores for normal or postinjury crypt cells are overlaid separately on the t-SNE projection shown in Fig. 4b. b CytoTRACE score for each cell. Cells are grouped and color coded on the basis of p57-Venus expression status and experimental condition (n = 567 postinjury p57-Venus⁺ cells, 1055 postinjury p57-Venus^‒ cells, 472 normal p57-Venus⁺ cells, and 923 normal p57-Venus^‒ cells from 2 biologically independent replicates). Bars indicate median values. ****P < 0.001 (Steel-Dwass test). c Representative phase-contrast images of sorted p57-Venus⁺ cell-derived organoids at 8 days after plating. Single cells were isolated from p57-DTR-Venus mice at 3 days after intraperitoneal injection of vehicle (left) or 5-FU at 150 mg/kg (right). Scale bars, 100 μm. d Efficiency of organoid formation from sorted single p57-Venus⁺ cells isolated from p57-DTR-Venus mice as determined at 8 days after plating as in c. Data are means + SEM (n = 3 mice). ***P < 0.005 (two-tailed Student’s t test). Source data are provided as a Source Data file.