Fig. 1: H. pylori infection reduces BMP signaling in Myh11+ myofibroblasts. | Nature Communications

Fig. 1: H. pylori infection reduces BMP signaling in Myh11+ myofibroblasts.

From: BMP feed-forward loop promotes terminal differentiation in gastric glands and is interrupted by H. pylori-driven inflammation

Fig. 1

a Confocal microscopy images of stomach antrum from Myh11CreErt2/Rosa26-mTmG mice. b Quantification of the signal overlap between α-SMA+ and Myh11-eGFP signal from Myh11CreErt2/Rosa26-mTmG mice (n = 3). c Heat map for selected genes expressed in sorted Myh11-eGFP cells from Myh11CreErt2/Rosa26-mTmG mice (n = 3). The gene expression level is presented as the read count. d Confocal microscopy images of gland hyperplasia (left) and Myh11+ myofibroblasts (right) in uninfected and 2-month H. pylori-infected Myh11CreErt2/Rosa26-mTmG mice. e Quantification of actin and Myh11 area per picture in uninfected (n = 3) and 2-month H. pylori-infected (n = 3) Myh11CreErt2/Rosa26-mTmG mice. f Heat map of RNAseq data with genes differentially expressed in uninfected (n = 3) and 2-month H. pylori-infected (n = 3) Myh11+ myofibroblasts. g Highlight of RNAseq data for differentially expressed genes involved in BMP signaling in uninfected (n = 3) and 2-month H. pylori-infected (n = 3) Myh11+ myofibroblasts. h qPCR validation of RNAseq data for BMP genes (uninfected: n = 3; infected: n = 3). Images are representative of at least three biological replicates. Scale bar: 100 µm. Data are presented as mean ± SEM. Statistical analyses were performed using Student’s t-test (two-tailed) for (e) and (h). RNAseq data are from three biological replicates per group. Source data are provided as a Source Data file.

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