Fig. 7: H. pylori induce an IFN-γ response.

a Immunofluorescence images of antrum tissue from uninfected (n = 3) and 2-month PMSS1 WT H. pylori-infected (n = 3) mice. Relative abundance of the Muc5ac+, Ki67+, and GSII+ compartments were quantified. b Representative images of H&E staining and gland height quantification of antrum tissue from C57BL/6 mice infected for two months with PMSS1 WT (n = 3) or ΔCagE H. pylori (n = 3). c Immunofluorescence images labeled for Ki67 and quantification of Ki67+ cells per gland in antrum tissue from C57BL/6 mice infected for two months with PMSS1 WT (n = 3) or ΔCagE H. pylori (n = 3). d Sm-ISH for Bmp2 and quantification of Bmp2 signal area per image in antrum tissue from C57BL/6 mice infected for 2 months with PMSS1 WT (n = 3) or ΔCagE H. pylori (n = 3). e GSEA with the hallmark of IFN-γ response genes for PMSS1 WT H. pylori-infected (n = 2) compared to uninfected C57BL/6 mice (n = 2). f qPCR for Ifnγ in the gastric antrum from C57BL/6 mice infected for 2 months with PMSS1 WT (n = 3) or ΔCagE H. pylori (n = 3). g Immunofluorescence images of antrum tissue from 6-week H. pylori-infected C57BL/6 mice (n = 4) and IfnγR KO mice (n = 4). Gland height of antrum tissue and relative abundance of the Muc5ac+, Ki67+, and GSII+ compartments were quantified. h CFUs from 6-week H. pylori-infected C57BL/6 mice (n = 4) and IfnγR KO mice (n = 4). i, j Sm-ISH images and quantification for Bmp2 and Id1 in antrum tissue of 6-week H. pylori-infected C57BL/6 mice (n = 4) and IfnγR KO mice (n = 4). Images are representative of at least three biological replicates. Scale bar: 100 µm, data are presented as mean ± SEM. Statistical analyses were performed using Student’s t-test (two-tailed) for (a–d, f–h, j). Source data are provided as a Source Data file.