Fig. 2: Overall structures of free S9.6 Fab and its complex with a 13-bp DNA-RNA hybrid.
From: Structural basis of R-loop recognition by the S9.6 monoclonal antibody
a Structure and cartoon schematic of free S9.6 Fab. Heavy chain (HC) is in blue; light chain (LC) is in orange. VH and CH1: variable and first constant domains of the heavy chain; VL and CL: variable and constant domains of the light chain. N and C termini are indicated. b Co-crystal structure of S9.6 Fab bound to a 13-bp hybrid of RNA (red) and DNA (green) strands. c Diagram of secondary structure and interactions of S9.6 with the hybrid. Nucleotides in direct contact are in a darker color. Hydrogen bonds are shown as dashed magenta lines; sugar–π packing interaction as parallel magenta lines. RNA 2′-hydroxyls in contact with S9.6 are shown as red spheres. Complementarity-determining regions (CDRs) H1–H3 and L1–L3 are denoted. The extended CDR-H3 loop hosts Y101-R104 residues for RNA strand recognition.
