Fig. 4: Recognition of the DNA strand by S9.6.
From: Structural basis of R-loop recognition by the S9.6 monoclonal antibody
a Two views of the S9.6 interface with the DNA strand (green). Hydrogen bonds are shown as black dashed lines; stacking or cation–π interactions as parallel magenta lines. b Schematic representation of S9.6 interaction with the DNA strand. c Fluorescence polarization titration analysis of a 13-bp hybrid binding by WT S9.6 and S9.6 variants harboring mutations at the DNA-binding interface. d Effects of S9.6 mutations on hybrid binding, calculated from c. e Fluorescence polarization titration analysis of a 13-bp hybrid binding by WT S9.6 and S9.6 variants at the Y54 site. f Effects of Y54 mutations on hybrid binding, calculated from e. *: no significant binding detected. Values are mean ± s.d. n = 3 biologically independent samples. Source data are provided as a Source Data file.
