Fig. 5: Effects of S9.6 and RNases H binding on hybrid helical geometry.

a–f Structural comparison between a DNA-RNA hybrid bound to S9.6 (RNA and DNA strands are in red and green), b Model dsDNA (blue), c Model dsRNA (magenta), d free hybrid (cyan, PDB 4WKJ⁴⁸), e hybrid bound to Bacillus halodurans RNase H1 (orange, PDB 1ZBL⁴⁹), and f hybrid bound to human RNase H1 (green, PDB 2QK9 (ref. ⁴⁵)). g Helical trajectory of the S9.6-bound hybrid (red) compared to that of a model dsRNA (black). h–l Structural superposition of the S9.6-bound hybrid onto the structures above (b–f), with h dsDNA, i dsRNA, j free hybrid, k hybrid bound to B. halodurans RNase H1, and l hybrid bound to human RNase H1. m Minor (top) and major (bottom) groove widths for nucleic acids shown in (a–f). Source data are provided as a Source Data file.