Fig. 1: The protein encoded by alr5059 shows a sequence similarity to atCyp38.

a Shown is the phylogenetic analysis of the sequences of the plant and cyanobacterial cyclophilin family as described in Materials and Methods (Supplementary Table 1). b The predicted domains of anaCyp40 are shown as bar diagram. The conserved domains were extracted from NCBI database (CDD)⁹¹ and additional regions for membrane anchoring or targeting predicted as described (Supplementary Table 2). TM: transmembrane region, DUF: domain of unknown function. c Purified anaCyp40_ΔTM-His (5 nM, blue) or BSA (5 nM, red) were incubated with 40 μM N-succinyl-ala-ala-pro-phe-p-nitroanilidine and the catalytic reaction monitored by the increase of absorption at 390 nm. Source data are provided as a Source data file. The values were normalized to the baseline and to the maximum. A representative experiment is shown. The average of the determine rate constant (n > 5 repetitions of the experiment) is presented and the standard deviation is shown as error bar. d E. coli BL21 (DE3) transformed with pET21a (upper panel) or pET21a-anaCyp40 (lower panel) were spotted on agar plates or plates containing the indicated concentrations of arsenic (upper left) or sodium chloride (lower right). Bacteria on normal plates were exposed to 50 °C or to UV-B (2.9 mWm⁻² nm⁻¹) for the indicated time. Representative images of growth after 24 h are shown.