Fig. 2: anaCyp40 is required for salinity stress response.

a Wild-type Anabaena sp. was grown in BG11 and cells were treated as indicated followed by RNA isolation and qRT-PCR analysis with specific oligonucleotides for anaCyp40 (Supplementary Table 4). The mean of the ratio to the mRNA abundance in non-treated cultures is shown and error bars indicate the standard deviation (n = 4). The statistical significance determined by ANOVA (Duncans) of the difference to non-treated cultures is indicated. b Two independently generated AFS-I-anaCyp40 strains and wild-type cells were spotted at two indicated concentrations at BG11 supplemented with indicated concentrations of NaCl. The plates were imaged after 8 days of growth. c Wild-type (blue) and AFS-I-anaCyp40 (orange) were grown in BG11 liquid medium (left) with additional 100 mM NaCl (right). The culture density was imaged after 8 days (top) and the average values and the standard deviation for the growth of three independent cultures normalized to the OD at the start of the analysis are shown (bottom). Source data are provided as a Source data file.