Fig. 3: anaCyp40 is important for the photosynthetic performance under high-light.

a, b Wild-type (blue) and AFS-I-anaCyp40 (orange) were grown in BG11 liquid medium at 120 µE illumination (right). The culture density was imaged after 8 days (a) and the average values for the growth of three independent cultures normalized to the OD at the start of the analysis are shown (b). c The chlorophyll content in wild-type (blue) and AFS-I-anaCyp40 (orange) cells grown under 40 µE or 120 µE (transparent color) was analyzed and normalized to cell density for three independent experiments. d, e The PC (d) and the APC (e) content in wild-type (blue) and AFS-I-anaCyp40 (orange) cells grown under 40 µE or 120 µE (transparent color) was analyzed and normalized to cell density. The statistical significance was analyzed and p < 0.001 is indicated. f The oxygen evolution rate was determined for wild-type (green) and AFS-I-anaCyp40 (orange) grown in BG11 liquid medium at 40 (dark color) or 120 µE illumination (transparent color). The average values and the standard deviation are shown. Stars indicate statistical significance with p < 0.001. g The photosynthetic parameters were determined by PAM. The Φ(II) for wild-type and mutant culture grown at 40 or 120 µE are shown. The statistical significance of the change was analyzed for same illumination condition and p < 0.001 is indicated. Error bars in b–g indicate standard deviation and the bare represents the mean. In b–g the statistical significance was analyzed by ANOVA (Duncans) and the star indicates a p < 0.001. h, i The 77 K fluorescence emission spectra of Anabaena sp. wild-type (green) or the insertion mutant of anaCyp40 (I-anaCyp40, orange) grown at normal (solid line) or high-light (dashed line) were recorded by excitation with 590 nm (f) or 440 nm (g). Normalization was done at 800 nm, and the spectra are the average of at least three independent biological replicates. The color and line coding shown in (f). Source data are provided as a Source data file.