Fig. 4: The impact of anaCyp40 on assembly of photosynthetic complexes.

a Membranes of wild-type (green) and AFS-I-anaCyp40 (orange) grown in BG11 liquid medium at 40 or 120 µE illumination were solubilized and subjected to BN-PAGE. The chlorophyll staining (left) and the Coomassie Blue staining (right) are shown. On the left migration of complexes and between the two images the migration of the molecular weight standards is show. The Coomassie Blue staining was quantified by ImageJ and the intensity of the individual complexes was normalized to the intensity in wild-type grown at 40 µE light intensity. b Phycobilisomes were isolated from cultures grown as in (a) and subjected to a 10–50% sucrose gradient. A representative profile is shown. c Equal amounts of phycobilisomes (5 µg protein) isolated from the strains as in (a) were subjected to SD-PAGE as indicated. The migration of the molecular weight is shown on the right and the proteins are assigned⁵⁴. d The strain AFS-anaCyp40-strep was grown in BG11, cells harvested and solubilized in low concentrated buffer and subjected on top of a 10–50% sucrose gradient. The fractions were subjected to SDS-PAGE followed by Western blotting (DB71-staining shown) and incubation with indicated antibodies. PBS and Thylakoid membrane fractions are indicated. e Cell lysate (CL) and phycobilisomes (PBS) were isolated in buffer with high concentration of phosphate from AFS-anaCyp40-strep and subjected to SDS-PAGE followed by Western blotting (DB71 staining shown) and incubation with indicated antibodies.