Fig. 4: Drug sensitivity and drug target correlations of childhood ALL cell lines.

a Heatmap depicting the sDSS of the 528 drugs from the DSRT across the 43 tested ALL cell lines. The x-axis is the cell lines ordered by the rank from hierarchical clustering (Pearson ward.D2) and the colors are the individual sDSS for each drug. The legends indicate the CLC and cytogenetic subtype of the cell lines. Higher sDSS indicates a more potent and effective drug relative to normal bone marrow cells. b Scatter plot of the median versus variance of the sDSS for each drug across the tested cell lines. Selected drugs are highlighted and colored according to their drug class. c The correlation between the sDSS for the glucocorticoids in the DSRT and relative protein level of the glucocorticoid receptor, NR3C1 for the 43 tested ALL cell lines. The x-axis shows the relative log2 protein level of NR3C1 and the y-axis shows the sDSS for each respective cell line (n = 43). The Pearson correlation coefficient (R) and two-sided t-distribution P values (P) for the comparison are shown in each plot. The linear regression trendline (black) and its 95% confidence interval (shaded gray area) are shown in the graph. The colors indicate the cytogenetic subtype of the cell lines, as annotated in 4e. d Scatter plot of the Spearman correlation versus rank for the sDSS of each drug and the relative log2 protein level of its putative protein target. The drugs are colored by drug classes and selected drug–drug target pairs are highlighted. e Ranked Pearson correlations of tacrolimus sDSS and highlighted proteins of the FKBP family (purple circles) and a scatter plot of tacrolimus sDSS and FKBP10 levels for each of the 29 tested ALL cell lines where FKBP10 was quantified. The x-axis shows the relative log2 protein level of the protein and the y-axis shows the sDSS for each respective cell line (n = 29). The Pearson correlation coefficient (R) and two-sided t-distribution P values (P) for the comparison are shown in each plot. The linear regression trendline (black) and its 95% confidence interval (shaded gray area) are shown in the graph. The colors indicate the cytogenetic subtype of the cell lines. Source data are provided as a Source Data File 4.