Fig. 1: Online targeted labelling of single functionally defined neurons.
From: Brain-wide projection reconstruction of single functionally defined neurons
a Four key steps for data collection and processing. b Left: Representative two-photon image in L2/3 of the AUD of an awake mouse. Two neurons were chosen and are marked with dashed coloured lines. Right: Ca2+ transients (neuron #1) responding to pure-tone stimulation (average of 8 trials for each of 6 intensities and 11 frequencies). Single-trial (grey) and trial-averaged (blue) Ca2+ transients are shown. c Colour-coded FRAs of the two neurons marked in (b). d Best frequency map of all the neurons in the imaging plane shown in (b). The colour code is on the right side. e Demonstration of single-cell electroporation. Briefly, pipettes containing OGB-1 and Cre-GFP plasmid were advanced towards the somata of neuron #1 and neuron #2. Then, the dye and DNA plasmids were electroporated into the neurons by applying trains of voltage pulses. f Monitoring of the fluorescence intensities of the two electroporated neurons by two-photon imaging on different days (day 3, day 10, and day 15) after electroporation.
