Fig. 5: Axons projecting to the contralateral AUD can be reliably labelled and reconstructed.

a The distance between the ipsilateral and contralateral AUDs is twice as long as that between the ipsilateral AUD and striatum. b Brain-wide distributions of AUD-projecting neurons achieved by a retrograde labelling strategy (AAV2/2-Retro-mRuby3). The detected somata were registered to the standard Allen Brain Atlas, as denoted by red dots. The white dashed arrow indicates the injection site. Three different views are shown (left: horizontal; upper right: sagittal; lower right: coronal). c Demonstration of single-cell electroporation. d Representative example showing two-photon imaging of two dual-colour labelled neurons (neurons #5 and #6) on day 7 after electroporation. e Left, reconstruction of two representative neurons (neurons #5–6; obtained from one mouse) labelled with a plasmid together with local AAV injection. Right, reconstruction of nine neurons (neurons #5–13; obtained from five mice) labelled with a plasmid with local AAV injection. f Reconstructed dendrites of the two representative neurons (top) in (e) (left) and all nine neurons (bottom) in (e) (right). g Left, reconstruction of two representative neurons (neurons #14–15; obtained from one mouse) labelled with a plasmid without nearby AAV injection. Right panel, reconstruction of nine neurons (neurons #14–22; obtained from five brains) labelled with a plasmid without nearby AAV injection. h Comparison of the success rates of axonal terminal filling in the contralateral AUD with a plasmid with AAV injection and without AAV injection (n = 9 neurons from 5 mice for each group).