Fig. 2: RCOR1 is enriched in accessible, transcriptionally permissive chromatin.

a Scheme depicting the MNase digestion of chromatin coupled to ultracentrifugation for chromatin accessibility assays. b Western blot analysis of the immunoprecipitation of RCOR1 on MNase-treated chromatin. Panels are representative of two independent experiments. c Western blot analysis of RCOR1 coimmunoprecipitated histone H3 modifications from MNased chromatin extracts. Panels are representative of two independent experiments. d Agarose gel showing the distribution of DNA fragments among the different fractions obtained from the ultra-centrifuged chromatin products. Bps: base pairs. STD: DNA Ladder. EtBr: Ethidium Bromide. This experiment performed once. e Western blot analyses of distribution of RCOR1, EZH2 and H3 in the sucrose gradient sedimentation equilibria. Upper arrows indicate standard molecular sizes resolved by this method. This experiment was performed once. f HT22 cells were stained with double immunolabeling of RCOR1 (green) and different histone modifications as shown in red. Right panels show magnified regions of original images highlighted inside dashed squares. Images are representative of three independent experiments. g Van Steensel’s plot of 2D colocalization between RCOR1 and each co-stained histone modification or DNA. CCF: Cross correlation function. h RCOR1 (blue) and H3K4me3 (green) or H3K27me3 (red) ChIP-seq positions mapped in 3D simulated chromosome 20 of human K562 cells based on Hi-C information as constraints. i Radial distribution function analysis of the 3D colocalization between RCOR1 and histone modifications. RDF: Radial distribution function. Source data are provided as a Source Data file.