Fig. 3: Loss of Chkb activity exerts a major effect on neutral lipid abundance. | Nature Communications

Fig. 3: Loss of Chkb activity exerts a major effect on neutral lipid abundance.

From: Mechanism of action and therapeutic route for a muscular dystrophy caused by a genetic defect in lipid metabolism

Fig. 3

Comparison of expression levels of major lipids between the Chkb+/+ and Chkb−/− mice. The analysis was performed on A, B, 12-day old hindlimb (quadriceps), and C, D, 30 days old hindlimb (quadriceps) samples. Each point is an individual lipid species (specific fatty acid composition). B, D Summary of fold change and statistical tests performed on major glycerophospholipids. A, C Boxplots bounds (hinges) correspond to the 25th and 75th percentiles of the data with the boxplot center corresponding to the median value. The upper/lower whiskers extend from the hinges to the largest/smallest value no further than 1.5 times the interquartile range away from the hinges. For A–D, n = 3 independent Chkb+/+ and 3 independent Chkb−/− mice. Pairwise Wilcoxon signed rank test with Bonferroni correction was used to determine the significance of a median pair-wise fold-increase in lipid amounts at an overall significance level of 5%. All statistical tests were two-sided. As the Bonferroni correction is fairly conservative, significant differences are reported at both pre-correction (*) and post-correction (***) significance levels. AcCa, acylcarnitine; TG, triacylglycerol; DG, diacylglycerol; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; PI, phosphatidylinositol; PS, phosphatidylserine. For (A, B), p = 0.0002 (AcCa), p = 0.0.2500 (DG), p = 0.0625 (TG), p = 0.1748 (PC), p = 0.0000 (PE), p = 0.1250 (PG), p = 0.0070 (CL), p = 0.0078 (PI), p = 1 (PS). For (C, D), p = 1 (AcCa), p = 0.0.0001 (DG), p = 0.0000 (TG), p = 0.5059 (PC), p = 0.0000 (PE), p = 0.6720 (PG), p = 0.2162 (CL), p = 0.1495 (PI), p = 0.0179 (PS). E Transmission electron microscopy (TEM) appearance of the hindlimb muscle samples (quadriceps) of Chkb+/+ and Chkb−/− mice at 12 days and 115 days of age. Images are representative of 3 mice per group with similar appearance. LD = Lipid droplets. M = Mitochondria. * = Disrupted sarcomeres. Scale bar = 1.5 μm. F Quadriceps muscle sections of 30 days old Chkb+/+ and Chkb−/− mice were fixed and stained with BODIPY-493/503 to visualize LDs (Green). Concanavalin A dye conjugate (CF™ 633) and DAPI were used to stain membrane (Red) and nucleus (Blue) respectively. Images are representative of 3 mice per group with similar results. Scale bar = 40 μm. Source data are provided as a Source Data file.

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