Fig. 5: Quantitative imaging of endogenous H2S in vivo.
From: A hydrogen sulphide-responsive and depleting nanoplatform for cancer photodynamic therapy
a PA images and b PA signals intensity of mouse livers at 680 and 900 nm, c Ratiometric PA signal PAS680/PAS900 against various treatments (i.p.) of L-Cys (0, 1, 2, 3, 4, and 5 mM, 100 μL) and i.v. injection of ZNNPs (10 mg/kg) after 30 min. Data are presented as mean ± s.d. (n = 5 independent mice). d Actual H2S concentration in mouse liver in a. e Derived equation for endogenous H2S quantification. f PA images, g Ratiometric PAS680/PAS900 signals and h H2S concentrations (fitting & actual) against various treatments (i.p.) of metformin (0, 1, 3, 5 mg in 100 μL PBS buffer) for 7 days and i.v. injection of ZNNPs (10 mg/kg). Data are presented as mean ± s.d. (n = 4 independent mice), data of “Actual” are presented as actual values (n = 2 independent mice). i Schematic description of ICH model construction and detection of endogenous H2S. j PA images, k PA intensity at 680 and 900 nm and l ratiometric PAS680/PAS900 signals of ICR mice with i.v. injection of ZNNPs (10 mg/kg) (n = 3). m Fluorescence images of mice brain slice in j. Statistical differences were analyzed by Student’s two-sided t-test. Source data are provided as a Source Data file.
