Fig. 6: ZNNPs@FA induces HCT116 cell death via intracellular H2S depletion.
From: A hydrogen sulphide-responsive and depleting nanoplatform for cancer photodynamic therapy
a Proliferation assays of HCT116 cells with different treatments (20 μg/mL ZNNPs@FA or ZNNPs@FA + 100 μM NaHS) for 4 h. b H2S concentration determination of HCT116 cells treated with different concentrations (0, 25, 50, 100 μg/mL) of ZNNPs@FA for 24 h. c GAPDH activity in various groups of cells. d ECAR quantification of cells with various treatments (50, 100 μg/mL ZNNPs@FA, or 20 μg/mL ZnCl2 for 6 h). e Mitochondrial membrane potential of HCT116 cells after 24 h of incubation with different concentrations (0, 10, 50, 75, and 100 μg/mL) of ZNNPs@FA measured by JC-1 staining. Scale bar = 50 μm. f Cell mitochondrial stress and g OCR determination of cells treated with various treatments (50, 100 μg/mL ZNNPs@FA, or 20 μg/mL ZnCl2) for 6 h. h The mechanism on H2S depletion inhibiting the proliferation of HCT116 cells. i Tumor growth profiles. j Immunofluorescence staining of tumorous tissues with antibodies against CD-31 and VEGF. Data are presented as mean ± s.d. (n = 3). Statistical differences were analyzed by Student’s two-sided t-test. Source data are provided as a Source Data file.
