Fig. 7: H2S-activated PDT effect of ZNNPs@FA in HCT116 cells.
From: A hydrogen sulphide-responsive and depleting nanoplatform for cancer photodynamic therapy
a Confocal fluorescence imaging and b quantification of H2S-activated ZNNPs@FA enabling 1O2 generation in HCT116 cells pretreated with PBS buffer, ZnCl2 (40 μg/mL, 10 min), NaHS (1 mM, 1 h), L-Cys (24 μg/mL, 1 h), PAG (50 μg/mL, 0.5 h)+L-Cys (24 μg/mL, 1 h), LPS (1 μg/mL, 6 h)+L-Cys (24 μg/mL, 1 h), or PAG (50 μg/mL, 0.5 h)+LPS (1 μg/mL, 6 h)+L-Cys (24 μg/mL, 1 h) upon 660 nm laser irradiation (50 mW/cm2, 3 min) using DCF-DA kit. c Mitochondrial membrane potential of cells treated with PBS buffer, ZNNPs@FA (12 h, 50 μg/mL), ZNNPs@FA (12 h, 50 μg/mL)+660 nm (50 mW/cm2, 3 min) using JC-1 dye. d Viability of HCT116 cells after 12 h of treatment with ZNNPs@FA (0, 0.1, 1, 10, 25, 50, and 100 μg/mL), or pretreated with ZnCl2 (300 μM) for 10 min measured by MTT assay. Data are presented as mean ± s.d. (n = 3). Statistical differences were analyzed by Student’s two-sided t-test. Source data are provided as a Source Data file.
