Fig. 1: Reconstitution of suPAR dimer and its binding to ATF.

a Recombinant suPAR formed into dimer at high concentration and low pH. suPAR was dissolved to 0.25 mg/mL concentration or 16 mg/mL concentration at low pH (pH 4.6) or neutral pH (pH 7.4) for 1 day, and was analyzed on a gel filtration column. b suPAR was separated into two peaks by ion-exchange chromatography, which was identified as monomer or dimer on gel filtration chromatography (insert). c Binding kinetics of the ATF, in 3-fold dilution, on suPAR monomer or dimer captured by anti-uPAR immobilized on CM5 chip by surface plasmon resonance. Sensorgrams recorded for the association (120 s), and dissociation (600 s) phases of the various ATF concentrations are shown as solid black lines with the corresponding curves fitting to a 1:1 Langmuir binding superimposed as solid red lines. The derived kinetic rate constants are shown in d. Source data are provided as a Source Data file.