Fig. 6: Transgenic CerS2 inactivation decreases plasma and muscle long-chain ceramides and muscle UPR gene expression. | Nature Communications

Fig. 6: Transgenic CerS2 inactivation decreases plasma and muscle long-chain ceramides and muscle UPR gene expression.

From: Long-chain ceramides are cell non-autonomous signals linking lipotoxicity to endoplasmic reticulum stress in skeletal muscle

Fig. 6

a Ceramides (Cer) 40:1 and Cer 42:1 in the soleus muscle of transgenic mice heterozygous (Het CerS2 H/A; grey) and homozygous (Homo CerS2 H/A; blue) for catalytically inactivated ceramide synthase 2 compared to wildtype (WT; red) (WT, Het CerS2 H/A n = 6; Homo CerS2 H/A n = 3; one-way ANOVA; WT vs Het CerS2 H/A, Cer 40:1 P = 0.03, Cer 42:1 P = 0.03; WT vs Homo CerS2 H/A, Cer 40:1 P < 0.0001, Cer 42:1 P = 0.0005). b Cer 40:1 and Cer 42:1 in the plasma of Het CerS2 H/A (grey) and Homo CerS2 H/A (blue) mice compared to WT (red) (WT, Het CerS2 H/A n = 6; Homo CerS2 H/A n = 3; one-way ANOVA; WT vs Het CerS2 H/A, Cer 42:1 P = 0.0018; WT vs Homo CerS2 H/A, Cer 40:1 P = 0.0006, Cer 42:1 P < 0.0001). c Activating transcription factor 4 (Atf4), Heat Shock Protein Family A (Hsp70) Member 5 (Hspa5) and ER Degradation Enhancing Alpha-Mannosidase Like Protein 1 (Edem1) soleus muscle UPR gene expression in Het CerS2 H/A (grey) and Homo CerS2 H/A (blue) mice compared to WT (red) (WT, Het CerS2 H/A n = 6; Homo CerS2 H/A n = 3; one-way ANOVA; WT vs Het CerS2 H/A, Atf4 P = 0.019, Hspa5 P = 0.02; WT vs Homo CerS2 H/A, Atf4 P < 0.0001, Hspa5 P = 0.019) *P ≤ 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Data are expressed as mean ± SEM with individual data points. Source data are provided as a Source Data file.

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