Fig. 1: Generation of behavior epoch-specific brain-wide activity maps. | Nature Communications

Fig. 1: Generation of behavior epoch-specific brain-wide activity maps.

From: Brain-wide mapping reveals that engrams for a single memory are distributed across multiple brain regions

Fig. 1

a Activity mapping pipeline. We used Fos-TRAP mice crossed with a Cre-dependent tdTomato reporter mouse line to prepare three behavioral cohorts: home cage, contextual fear conditioning (CFC), and fear memory recall (Recall). Brain samples were used for SHIELD processing, SPIM imaging, 3D reconstructions, automatic brain region segmentation, and automatic single activated-cell detection. Primary somatosensory cortex (S1 Ctx), corpus callosum (cc), caudate putamen (CPu). b Brief, representative version of our brain-wide activity mapping results (n = 7 home cage mice, n = 10 CFC mice, n = 9 Recall mice), where the behavioral groups are normalized to home cage data. Individual values indicate fold increases in the numbers of activated neurons relative to home cage. Primary visual area (V1), primary somatosensory area barrel field (S1BF), orbital cortex (OL), prelimbic cortex (PrL), infralimbic cortex (IL), entorhinal cortex (EC), anterior cingulate cortex (CgA), olfactory cortex (Ol), retrosplenial cortex (RSA), basolateral amygdala (BLA), central amygdala (CeA), mediodorsal thalamus (MD), dorsal geniculate thalamus (GD), anteromedial thalamus (AM), anterodorsal thalamus (AD), anteroventral thalamus (AV), ventroposterior medial nucleus of thalamus (VPM), nucleus reuniens of thalamus (RE), nucleus accumbens (ACB), ventromedial hypothalamus (VMH), paraventricular hypothalamus (PVN), lateral hypothalamus (LH), mammillary body (MB), dentate gyrus (DG), hippocampal CA1 (CA1), hippocampal CA3 (CA3), para-subiculum (ParaS), pre-subiculum (PreS), bed nucleus of the stria terminalis (BNST), and periaqueductal gray (PAG). For a full list of brain regions analyzed, refer to Supplementary Tables 1 and 2. c 3D rendering of four brain regions, specifically S1BF, BLA-LA, anterior group of dorsal thalamus, and hippocampus, showing automatically segmented activated neuronal populations along with corresponding heat maps. LA lateral amygdala, D dorsal, V ventral, A anterior, P posterior, M medial, L lateral.

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