Fig. 3: Brain-wide engram reactivation.

a To identify CFC activated neurons that are reactivated by recall (i.e., exhibiting overlap), activated neurons during CFC were labeled using 4-OHT on day 1 and three days later activated neurons during recall were labeled using cFos staining. b, c Rank-ordered list of 88 brain regions with their engram reactivation values (percentage of CFC activated neurons that subsequently showed recall-induced cFos) that passed our statistical criteria (n = 9 CFC mice). For a list of 159 non-significant brain regions based on our statistical criteria, refer to Supplementary Table 3. Regions highlighted in gray are consistent with those identified by the brain-wide engram index analysis in Fig. 2. Regions with an asterisk were tested in the optogenetic engram reactivation behavioral experiments described in Fig. 4.