Fig. 2: Evaluation of in vivo TAM depletion and T cell infiltration after PLX-NP@Gel treatment in recurrent B16F10 tumor model. | Nature Communications

Fig. 2: Evaluation of in vivo TAM depletion and T cell infiltration after PLX-NP@Gel treatment in recurrent B16F10 tumor model.

From: Depletion of tumor associated macrophages enhances local and systemic platelet-mediated anti-PD-1 delivery for post-surgery tumor recurrence treatment

Fig. 2

a, b Representative flow cytometry plots of TAMs (a) and CD8+ T cells (b) in the recurrent tumor tissues after treatments with saline, NP@Gel, PLX, PLX-NP, and PLX-NP@Gel. c–e Quantitative analysis of intratumoral densities of F4/80+ macrophages (c, PLX-NP@Gel vs. PLX-NP: *P = 0.0358; PLX-NP@Gel vs. PLX: **P = 0.0055), CD8+ T cells (d, PLX-NP@Gel vs. PLX-NP: *P = 0.0242; PLX-NP@Gel vs. PLX: *P = 0.0147), and IFNγ+ CD8+ T cells (e, PLX-NP@Gel vs. PLX-NP: **P = 0.0026; PLX-NP@Gel vs. PLX: ***P = 0.0007) per tumor mass in the recurrent tumor tissues after treatments with saline, NP@Gel, PLX, PLX-NP, and PLX-NP@Gel. Data are presented as mean ± s.d. (n = 5 biologically independent samples) and analyzed with one-way ANOVA followed by Dunnett’s multiple comparison test. f, g Representative confocal microscopy images of immune-stained TAMs (f) and CD8+ T cells (g) in the saline group and PLX-NP@Gel group (n = 3 biologically independent samples). Scale bar, 100 µm. The experiments were repeated three times. Source data are provided as a Source Data file.

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