Fig. 3: Cryo-EM structure of N1-CA09-sNAp-155.
From: Structure-based design of stabilized recombinant influenza neuraminidase tetramers
a Cryo-EM 2D class averages for N1-CA09-sNAp-155 showing fully closed tetramers (scale bar, 10 nm) (top left). Cryo-EM map, colored by individual head domain, viewed down the four-fold symmetry axis (top right) and an orthogonal view (bottom left). b The three major regions targeted for mutation: WT N1-CA09 (PDB 4B7Q, left), with residues targeted for mutation indicated (red). Space A (top), Space B (middle), and space D (bottom). Corresponding regions in the N1-CA09-sNAp-155 single-particle reconstruction (right), with mutated residues indicated (blue) and non-mutated residues involved in packing colored by chain as in panel a. The red sphere highlights the position of a cavity in space D.
