Fig. 2: Repertoire analyses of heavy chain variable regions in TC-mAb mice. | Nature Communications

Fig. 2: Repertoire analyses of heavy chain variable regions in TC-mAb mice.

From: Efficient human-like antibody repertoire and hybridoma production in trans-chromosomic mice carrying megabase-sized human immunoglobulin loci

Fig. 2

a Human heavy chain gene utilization in hPBMCs from healthy donors and in TC-mAb mice with (female, 24 weeks old) and without (five female mice, 18 weeks old) immunization. Percentage frequency use of V gene segments in healthy human donors (grey), TC-mAb mice with (green) and without immunization (blue) are represented. b Circos plots comparing VDJ gene association. The gene segments are grouped as subfamilies and are shown together with the first digit of their allele name. Links indicated the relative frequencies of specific VDJ combinations, and wider links indicate higher frequencies of recombination. The relative CDRH3 length (c), P and N nucleotide addition at joining positions (d), and amino acid usage (e) within the CDRH3 of productive rearrangements.

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