Fig. 4: Somatic hypermutation analysis.

The frequency of SHM at every position of the variable region was calculated as described in the Methods section and is represented for VH and VK chains in OVA-immunized TC-mAb mice (a, b) and unimmunized TC-mAb mice (c, d) (same as Fig. 2). e The phylogenetic tree of the clone type CLH001. The circular dendrograms of the 20 most frequently used clone lineages were over-laid with the number of copies carrying the same CDRH3 sequences. The leaves of circular dendrograms have a maximum of 50 reads; therefore, as the number increases, the circle in the leaves increases. The colour of the circle indicates with (red) or without (blue) immunization in TC-mAb mice. The amino acid sequences of 12 clonotypes containing more than 50 copies in the CLH001 clone lineage. The clonotype numbers and each copy number are also indicated. f Amino acid sequence alignment of the 12 clonotype variable regions of CLH001.