Fig. 7: Analysis of B cell development.

a Flow cytometry of B cell subsets of early-developing B and mature B cell compartments in the bone marrow of 20–22-week-old ICR and TC-mAb mice. Panels indicate Pro-B/ Pre-B (IgM⁻B220⁺), immature B (IgM⁺B220⁺) and mature recirculating B (IgM^lo/+B220^hi) cells in bone marrow. Gating strategies are presented in Supplementary Fig. 15. b Flow cytometry of lymphocyte (left), mature (centre) and mouse/human Igk⁺ immature B cell compartments. Gating strategies are presented in Supplementary Fig. 17. c The percentage of follicular (CD19⁺CD93⁻CD21⁺CD23⁺), and marginal zone (CD19⁺CD93⁻CD21^hiCD23⁻) B cells in the mature B cells and that of transitional 1 (IgM^hiCD23⁻), 2 (IgM^hiCD23⁺) and 3 (IgM^loCD23⁺) B cells in the Igκ⁺ immature B cells of spleen (n = 5) and lymph node (n = 5/4 (ICR/TC-mAb)) are presented. Eight to 9-week-old unimmunized mice were analysed. All gating panels of B cell subsets are presented in Supplementary Figs. 18 and 20. P-values between ICR and TC-mAb mice of the percentages of B cell subsets were 0.115 (splenic follicular), 0.560 (marginal zone), 0.016 (transitional 1), <0.001 (transitional 2), 0.009 (transitional 3) and 0.301 (lymph nodus follicular). Box plots are indicated in terms of minima, maxima, centre, bounds of box and whiskers (1.5 interquartile range value), and percentile in the style of Tukey. *P < 0.05 (two-tailed unpaired Student’s t test). The number of follicular and marginal zone B cells in the spleen mature B cells (d) and B1a (CD19⁺B220^loCD5⁺CD43⁺) in spleen and B1a/b and B2 in PECs (e) at 6, 8 and 20 weeks-age of unimmunized mice are represented. All gating panels of B cell subsets are presented -n Supplementary Figs. 21–26. P-values of the cell number of B cell subsets between ICR and TC-mAb are indicated in the Source Data file. Data are presented as means. Error bars indicate the standard deviation of triplicate measurements. Results were pooled from two independent experiments. *P < 0.05 (two-tailed unpaired Student’s t test).