Fig. 9: Analysis of spleen cells in repeatedly immunized mice for monoclonal antibody production. | Nature Communications

Fig. 9: Analysis of spleen cells in repeatedly immunized mice for monoclonal antibody production.

From: Efficient human-like antibody repertoire and hybridoma production in trans-chromosomic mice carrying megabase-sized human immunoglobulin loci

Fig. 9

a Representative images of fluorescence immunohistochemistry. The spleens of OVA-immunized TC-mAb mice were harvested and analysed by immunohistochemistry. Sections were stained for follicular dendritic cells (CD35, red), GC B cells (GL7; green) and nuclei (DAPI, blue). The images were taken at ×20 magnification. All scale bars indicate 100 μm. Representative figure from two independent experiments is shown. b Flow cytometry of plasmablasts/plasma cells (CD138+TACI+). Each spleen sample was collected after the seventh and final booster steps of immunization (in mice of ~21 weeks old). Gating strategies and all gating panels of plasmablast and plasma cell subsets are presented in Supplementary Figs. 38 and 39.

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