Fig. 1: MitoTam improves metabolic parameters in naturally aged mice.
C57BL/6 mice 2 (young) and 18 months of age (aged) were treated i.p. once per week for a period of 4 weeks with MitoTam (2 mg/kg body weight; MT) dissolved in 4% EtOH in corn oil or with the vehicle (CO). a, b Lungs (a; young n = 8, aged n = 10, aged+MT n = 8; young vs. aged p = 0.005, aged vs. aged+MT p = 0.049) and VAT (b; n = 7; young vs. aged p = 0.004, aged vs. aged+MT p = 0.006) were excised and assessed for SA-β-gal activity, which was expressed as % of SA-β-gal-positive tissue. c–e Expression of p21waf1 mRNA in lungs (c; young vs. aged p = 0.005, aged vs. aged+MT p = 0.048), liver (d; young vs. aged p = 0.001, aged vs. aged+MT p = 0.035; c, d young n = 8, aged n = 11, aged+MT n = 8) and VAT (e; n = 7) was estimated by qRT-PCR. f Fasting glucose level in young (n = 6), aged (n = 8) and aged mice treated with MitoTam (n = 8) was assessed after 16 h of fasting (young vs. aged p = 0.021, aged vs. aged+MT p = 0.054). g Level of leptin in plasma was estimated (n = 9; young vs. aged p = 0.007, aged vs. aged+MT p = 0.026). For all panels, a–g one-way ANOVA, Tukey´s comparison multiple test was used. Data are expressed as mean ± SEM; *p < 0.033; **p < 0.002; ***p < 0.001. Source data are provided as a Source Data file.
