Fig. 4: RNA QASeq for gene expression level quantitation.

a Quantitation accuracy validation with ERCC spike-in reference sample. b RNA QASeq quantitation reproducibility in technical replicates. 10 ng total liver RNA was used as input. c Multiple amplicons per gene reduced quantitation variability in technical triplicate analysis of total liver RNA sample. The standard deviation for relative expression level in triplicate experiments became lower as the number of amplicons per gene increased from 1 to 5. Only the amplicons at 5′ end of mRNA were used when analyzing a subset of amplicons. d RNA relative expression level quantitation side-by-side comparison using four different methods in one breast cancer FFPE RNA sample. e Relative expression level measured by RNA QASeq in four clinical FFPE tumor tissue samples and three normal placenta FFPE samples. Hierarchical clustering indicated the expression patterns were the most similar between normal placenta samples.