Fig. 3: Rescue of cone-mediated visual function in rd12Gnat1−/− mice after ABE treatment. | Nature Communications

Fig. 3: Rescue of cone-mediated visual function in rd12Gnat1−/− mice after ABE treatment.

From: In vivo base editing rescues cone photoreceptors in a mouse model of early-onset inherited retinal degeneration

Fig. 3

a Breeding strategy to generate a homozygous rd12Gnat1−/− mouse line. Knockout (KO) of Gnat1 nearly completely abolishes the phototransduction signaling cascade from rods, leaving cone-mediated phototransduction. b Progression of cone degeneration in the rd12Gnat1−/− mouse from 2 weeks of age to 8 weeks of age. M-opsin, green. S-opsin, purple. Scale bar, 1 mm. c Experimental timeline to evaluate cone function and survival in rd12Gnat1−/− mice after ABE treatment. d Representative photopic ERG waveforms of M-cones (top) and the average b-wave amplitudes (bottom), evoked with the indicated green light flashes in treated and untreated rd12Gnat1−/− mice (n = 8, each group). Mean ± SD. ***P < 0.001; two-tailed Mann–Whitney U-test. P values are 0.08 at −0.5 and 0.0002 at 0.5, 1.5, and 2.5 light intensities. e Representative photopic ERG waveforms of S-cones (top) and the average b-wave amplitudes (bottom), evoked with the indicated UV light flashes, in treated and untreated rd12Gnat1−/− mice (n = 8, each group). Mean ± SD. ***P < 0.001; two-tailed Mann–Whitney U-test. P values are 0.08 at −1 and 0.0002 at 0, 1 and 2 light intensities. Source data are provided as a Source data file.

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