Fig. 5: Protection of cone photoreceptors and correct opsin localization in 2-month-old rd12Gnat1−/− following ABE treatment. | Nature Communications

Fig. 5: Protection of cone photoreceptors and correct opsin localization in 2-month-old rd12Gnat1−/− following ABE treatment.

From: In vivo base editing rescues cone photoreceptors in a mouse model of early-onset inherited retinal degeneration

Fig. 5

a Representative retinal flatmounts from untreated (left) and treated (right) rd12Gnat1−/− mouse eyes, labeled with M-opsin (green) and S-opsin (purple) antibodies. Scale bar, 1 mm. b Magnified view of M-cones (green) in the dorsal retina from untreated and treated rd12Gnat1−/− mice. Scale bar, 50 μm. c Magnified view of S-cones (purple) in the ventral retina from untreated and treated rd12Gnat1−/− mice. Scale bar, 50 μm. d Quantification of M-cones and S-cones in each field as shown in b, c, at dorsal and ventral regions of the retina, 1 mm away from the optic nerve. Five fields across the dorsal or ventral retina were analyzed from each eye, with a total of 20 fields from 4 eyes per group. Mea ± SD. **P < 0.01; ***P < 0.001; unpaired t-test, two-tailed. P values for dorsal and ventral M-cones and dorsal and ventral S-cones are 0.000001, 0.006, 0.00009, and <0.000001, respectively. e Retinal cryosections from Gnat1−/−, and untreated and treated rd12Gnat1−/− mice, showing the dorsal region labeled with M-opsin (top) and the ventral region labeled with S-opsin (bottom). DAPI, blue; OS outer segment, IS inner segment, ONL outer nuclear layer. Scale bar, 20 μm. Source data are provided as a Source data file.

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