Fig. 6: Single-cell RNA-seq of ABE-treated retinas reveals the rescue of genes associated with phototransduction and cone survival.

a Uniform manifold approximation and projection (UMAP) of the single-cell RNA-seq dataset colored by annotated cell type. b UMAP plots showing the distribution of single cells of 2-month-old WT, and untreated and treated rd12 retina samples (n = 4, each group). c Violin plot profiles of S-opsin (Opn1sw) and M-opsin (Opn1mw) expression levels in individual cone cells. d Violin plot profiles of cone phototransduction-associated expression levels in individual cone cells. ***P < 0.001; two-tailed Wilcoxon rank-sum test in Seurat. P values for c, d are provided in Supplementary Table 1. e Retinal cryosections showing the expression of cone-arrestin (purple) in 2-month-old WT, and untreated and treated rd12 mice. Cone cells are labeled with peanut agglutinin (PNA; green). DAPI, blue. Scale bar, 20 μm.