Fig. 4: ComFC binds single-stranded DNA and promotes its chromosomal integration.
From: ComFC mediates transport and handling of single-stranded DNA during natural transformation
a and b Transformation frequencies after electroporation with a single-stranded DNA (75-mer) coding for streptomycin resistance as donor DNA. Bars correspond to the average and standard deviation from the number of experiments indicated within the bars. * Assay detection limit as determined considering one colony obtained in a recA mutant. c Selective affinity of ComFC for single-stranded DNA. Indicated concentrations of His6-ComFC were incubated with a Cy5-labelled single- or double-stranded DNA oligonucleotide. The nucleoprotein complexes were resolved by native-PAGE. d Protection against nuclease activity. 78 nt long fluorescent oligonucleotide was incubated 50 min with 5 units of Mung Bean Nuclease (MBN) after 20 min of preincubation with drSSB or ComFC at the indicated concentrations. Products were analysed on a native polyacrylamide gel. Blots are representative of two experiments. Source data are provided as a Source Data file.
