Table 1 Data collection and structure refinement statistics.
From: ComFC mediates transport and handling of single-stranded DNA during natural transformation
Data collection | PRPP-bound B2-HpComF ¤ |
|---|---|
Space group | P1 |
Unit cell parameters | a = 58Å b = 88Å c = 123Å |
α = 80° ß = 76° γ = 76° | |
Wavelength (Å) | 0.979 |
Resolution range (Å)† | 46.5–2.5 (2.6–2.5) |
Before STARANISO | |
Measured/Unique reflections † | 895493/77488 (57841/5398) |
Completeness (%)† | 98.4 (92.6) |
Anomalous completeness (%)† | 95.0 (84.8) |
I/σ(I)† | 15.7 (0.6) |
After STARANISO | |
Measured/Unique reflections † | 752919/63154 (44297/3158) |
Completeness (%)† | 93.5 (94.2) |
Anomalous completeness (%)† | 90.6 (92.7) |
I/σ(I)† | 19.2 (1.6) |
Redundancy † | 11.9 (14.0) |
Anomalous redundancy † | 6.1 (7.1) |
CC1/2 † | 0.999 (0.387) |
CCano † | 0.715 (0.403) |
|DANO|/σ(DANO) | 1.404 (0.814) |
Rmerge (%)† | 8.8 (140.1) |
Rpim (%)† | 2.7 (38.3) |
SAD phasing | |
Number of sites | 12 |
Overall FOM | 0.39 |
Refinement | |
Resolution range (Å)† | 46.5–2.5 (2.6–2.5) |
Number of work/test reflections | 63118/3156 |
R/Rfree (%)† | 22.1/24.5 (25.6/27.2) |
Geometry statistics | |
Number of atoms | |
Protein | 12672 |
Ligand/ion | 184 |
Water | 282 |
r.m.s. deviations from ideal values | |
Bond lengths (Å) | 0.007 |
Bond angles (°) | 0.87 |
Average B-factor (Å2) | |
Overall | 69.74 |
Protein | 69.92 |
Ligand/ion | 79.81 |
Water | 54.88 |
Ramachandran plot | |
Favoured (%) | 97 |
Allowed (%) | 3 |
Outliers (%) | 0 |
