Fig. 4: iPEAQ biosensing with intermittent calibration. | Nature Communications

Fig. 4: iPEAQ biosensing with intermittent calibration.

From: Absolute measurement of cellular activities using photochromic single-fluorophore biosensors and intermittent quantification

Fig. 4

a Comparison of the irradiation procedure of PEAQ biosensing using repeated photochromism cycles (left), and iPEAQ biosensing using intermittent photochromism cycles (right). b Schematic overview of the intermittent calibration methodology. c Fraction of the light dose that can be saved using iPEAQ as a function of the number of intensiometric acquisitions per photochromism cycle. d Fluorescence intensity F0 (black) and \(\left[{{{{{{{{\rm{Ca}}}}}}}}}^{2+}\right]\) trace (green) of a single GCaMP6s-Q-expressing HeLa cell after stimulation with 25 μM histamine (black).

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