Fig. 4: Translation kinetics from coarse-grained HMM-results.
From: Direct measurements of mRNA translation kinetics in living cells
a, b Estimated occupancy and dwell-time of ribosomal subunits in the mRNA-bound state or the freely diffusive state for different HMM fitting model sizes. Full models (Supplementary Data 4â7) were coarse-grained into two states: mRNA-bound or free, using the threshold 0.25â”m2/s. c, d Weighted averages of occupancy and dwell-time, based on model sizes 7â11 (as shown in panels a, b, Supplementary Data 12). Error bars with caps represent weighted standard deviation, calculated from individual model sizes (7â11) with bootstrap estimated standard errors (shown as error bars without caps). nâ=â179569, 115349, 161623, and 191950 trajectory steps collected from 3, 3, 7, and 6 independent experiments for L9, H98, S2, and h6 labelling, respectively. Source data are provided as a Source Data file.
