Fig. 5: Inhibition of initiation by AtaT toxin and KSG.

a, b HMM-estimated dwell-time of 30S and 50S subunits in the freely diffusing state (a) or in the mRNA bound state (b) in presence or absence of 20 µg/ml KSG or during the expression of the toxin AtaT. The data shows weighted averages from coarse-grained HMM-fitted model sizes 7–11 (Supplementary Data 12). Results for individual model sizes (2–11) are shown in Supplementary Data 4, 17−21. Error bars with caps represent weighted standard deviation, calculated from individual model sizes (7–11) with bootstrap estimated standard errors (shown as error bars without caps). n = 161623, 141242, 101518, 179569, 182163, and 103391 trajectory steps collected from 7, 2, 5, 3, 2, and 5 independent experiments for 30S, 30S+KSG, 30S+AtaT, 50S, 50S+KSG, and 50S+AtaT, respectively. Source data are provided as a Source Data file.