Fig. 6: Estimated mRNA-bound-state dwell-time of 50S subunits at different temperatures and with microscopy data acquired at 30 or 60 ms camera exposure times.
From: Direct measurements of mRNA translation kinetics in living cells
The data shows weighted averages from coarse-grained HMM-fitted model sizes 7–11 (Supplementary Data 12). Results for individual model sizes (2–11), are shown in Supplementary Data 4 and 20−24. Error bars with caps represent weighted standard deviation, calculated from individual model sizes (7–11) with bootstrap estimated standard errors (shown as error bars without caps). n = 142400, 146595, 144083, 155262, 179569, and 93809 trajectory steps collected from 2, 2, 2, 3, 3, and 2 independent experiments for 30 and 60 ms frame time at 25 °C, 30 °C, and 37 °C respectively. Source data are provided as a Source Data file.
