Fig. 7: Translation kinetics of modified 30S subunits.

Estimated occupancy (a) and dwell-time (b) of tracked 30S subunit variants in the mRNA-bound state or the freely diffusive state. The data shows weighted averages from coarse-grained HMM-fitted model sizes 7–11 (Supplementary Data 12). Results for individual model sizes (2–11) are shown in Supplementary Data 7 and Supplementary Data 25−28. Error bars with caps represent weighted standard deviation, calculated from individual model sizes (7–11) with bootstrap estimated standard errors (shown as error bars without caps). n = 191950, 90335, 33838, 86221, and 55186 trajectory steps collected from 6, 6, 2, 2, and 2 independent experiments for 30S, O-ASD 30S, O-ASD-C722-A723 30S, O-30S, and O-30S-U1400, respectively. Source data are provided as a Source Data file.