Fig. 2: PD-L1 and ICOSL expression on mature blood cDC are exclusive and PD-L1high DC overexpress PVR, Nectin2, CD54, CD40, and PD-L2.
A Methods for the in vitro analysis of primary blood cDC. B Expression of PD-L1(x) vs ICOSL(y) on cDC at H24. 154 individual tests were annotated according to their expression of PD-L1 as high/low and ICOSL high/low with the thresholds of specific mean fluorescence intensity (MFI) at 3500 and 1000, respectively. C T-SNE of the 29 surface markers colored by stimuli (left), PD-L1 specific MFI (center), and ICOSL-specific MFI (right) using Qlucore software, n = 154. D Heatmap representing the expression of the 29 surface markers in the four groups defined by PD-L1 and ICOSL in “B”, and in Medium condition. Multigroup comparison by Kruskal–Wallis test and Tukey post-hoc test. Only the variables significant at a p-value < 0.05 are represented and ordered by increasing q-value (max q-value = 0.046), among 130 individual experiments, ordered as in Fig. 3A. E Correlation of PD-L1 (x) with PVR, Nectin2, CD54, PD-L2, and CD40 (y). ≪r≫ values are Spearman correlation coefficients, p-value are for two-sided statistical analyses, n = 154. TSLP thymic stromal lymphopoietin, Flu influenza, HKCA heat-killed Candida albicans, HKLM heat-killed Listeria monocytogenes, HKSA heat-killed Staphylococcus aureus, LPS lipopolysaccharide.
