Fig. 3: PD-L1 and ICOSL expression pattern characterize Secretory and Helper cDC.

A Heatmaps representing the cytokines and chemokines secreted by the cDC measured in H24 supernatants (top), and the CD4 Th cell cytokines measured after co-culture (bottom) in the 4 groups defined by PD-L1 and ICOSL expression and Medium condition. Only the variables significant at a p-value < 0.05 after Kruskal–Wallis test on the 5 groups and Tukey post-hoc tests are represented and ordered by increasing q-value (max q-value = 0.035 (top) and 0.055 (bottom)), among 130 individual experiments, ordered as in Fig. 2D. Cells in gray are missing values. Abbreviations for the perturbators: see Fig. 2. B Quantification of cytokines and chemokines secreted by the DC (top row) and of the CD4 Th cell cytokines (2 bottom rows) in the Medium (n = 23), PD-L1^high ICOSL^low (n = 38), and PD-L1^low ICOSL^high (n = 40) conditions (two-sided Kruskal–Wallis test on the 3 groups and Dunn’s multiple comparison test). Central line represents median, box represents quartiles, whiskers represent min to max. p values are represented by range: *<0.05, **<0.01, ***<0.001, ****<0.0001. NS not significant. MED medium, Hi high, Lo low. C Quantification of cytokines secreted by the CD4 Th cytokines after co-culture with pure cDC2 sorted as in Supplementary Fig. 5A and treated with R848 (Secretory) or Thymic stromal lymphopoietin (TSLP) (Helper, n = 3). For the R848-cDC2 the co-culture was performed in the presence of PD-1; IL-10; IL-10R; IL-4R; TIGIT multiple blockings “R848_block” or the corresponding isotypes “R848_Iso” (n = 7, two-sided Wilcoxon test). Bar represents median. Source data are provided as a Source Data file.