Fig. 4: RNAseq of tumor and blood cDC2 confirms that T-cell-inflamed HNSCC are infiltrated by Secretory DC. | Nature Communications

Fig. 4: RNAseq of tumor and blood cDC2 confirms that T-cell-inflamed HNSCC are infiltrated by Secretory DC.

From: PD-L1 and ICOSL discriminate human Secretory and Helper dendritic cells in cancer, allergy and autoimmunity

Fig. 4

A Analysis of differentially expressed genes (DEG) by DESeq2 between HNSCC tumor (n = 6) and blood cDC2 (n = 3). B Analysis of DEG from dataset GS87442 by DESeq2 between unstimulated cell and pRNA, a TLR7/8 ligand (left) or GM-CSF (center) and pRNA vs GM-CSF (right). C Venn diagram of upregulated genes identified in “B”. The blue and the yellow-colored area contain the genes of the Secretory and Helper signatures, respectively. D Venn diagram of the 639 tumor cDC2 upregulated genes with the Secretory and Helper signatures defined in “C”. E Supervised analysis of the 135 genes shared between tumor and pRNA “secretory” signature (light blue), 440 tumor specific genes (black), and the 64 genes shared between tumor and GM-CSF (yellow), using 3 gene lists: checkpoint and maturation markers (left, 148 genes), cytokines and chemokines (center, 169 genes), NFkB pathway (right, 100 genes). F Expression of selected genes in cDC2 from tumors and blood of HNSCC patients, central lines represent mean.

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