Fig. 3: Elucidation of the modification moieties on glycans. | Nature Communications

Fig. 3: Elucidation of the modification moieties on glycans.

From: Glyco-Decipher enables glycan database-independent peptide matching and in-depth characterization of site-specific N-glycosylation

Fig. 3

a Mass histogram of glycans in glycopeptides of the mouse liver dataset. The glycan masses were obtained by the glycan database-independent pipeline in Glyco-Decipher. Blue: PSMs with glycan mass that matched GlyTouCan glycans; Orange: PSMs with glycan mass that could not be annotated by GlyTouCan glycans. The bin width was set to 1 Da and centered at integer values. b A monosaccharide stepping method was designed for the deduction of the composition and the mass of modification moiety on modified glycans. c Percentage of PSMs with glycan parts annotated by database glycans and modified glycans. d Mass values of the ten most abundant modification moieties on glycans from glycopeptide spectra of the five mouse tissues. e Mass profiles of the modification moieties with known chemical compositions on modified glycans. f Examples of mass profiles of glycan modification moieties with unannotated composition. Source data are provided as a Source Data file.

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