Fig. 5: Quantitative analysis of site-specific glycosylation on prosaposin by Glyco-Decipher. | Nature Communications

Fig. 5: Quantitative analysis of site-specific glycosylation on prosaposin by Glyco-Decipher.

From: Glyco-Decipher enables glycan database-independent peptide matching and in-depth characterization of site-specific N-glycosylation

Fig. 5

Relative abundance distribution of glycans at each glycosite in prosaposin across five mouse tissues (heat map). Compositions and possible structure illustration of the high-abundance glycans are annotated at the top. For a more intuitive demonstration, the abundance distributions of glycans at each glycosite are listed in the right radial diagrams. In each radial diagram, nodes around the circle denote glycans linked to prosaposin, and donuts in the center denote glycosites identified in prosaposin. Linkage between the node and the center donut indicates that the glycosite was modified by the corresponding glycan. The percentage value in each donut indicates the relative abundance for a certain type of glycan. All M6P identifications were validated by the diagnostic oxonium ion (phosphorylated hexose, m/z = 243.0269) in glycopeptide spectra. H Hex, N HexNAc, A NeuAc, G NeuGc, F Fuc, P Phosphorylation. See Supplementary Data 7 for detailed information of glycan nodes.

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