Fig. 5: Mechanical strain reversibly regulates innate immune response in alveolus chip in uninfected chips.
a Volcano plot of DEGs comparing epithelial cells from alveolus chips under static or 5% strain culture condition for 4 days. DEGs (Padj < 0.05) with a fold change >1.5 (or <−1.5) are indicated in red. The names of DEGs belonging to the innate immune pathway are labeled. b Dot plot showing the biological processes activated or suppressed by 5% strain vs. static culture condition in alveolus chips from a. c, d Volcano plots of DEGs showing the effects of switching 5% strain to static for 2 days on epithelial cells on-chip (c) and the effects of increasing 5% strain to 10% strain for 2 days on epithelial cells on-chip (d). e Heat map showing differentially expressed innate immune genes in epithelial cells under different magnitudes of mechanical strains. f, g Volcano plots of DEGs showing the effects of switching 5% strain to static for 2 days on endothelial cells on-chip (f) and the effects of increasing 5% strain to 10% strain for 2 days on endothelial cells on-chip (g). h Heat map showing differentially expressed innate immune genes in endothelial cells under different magnitudes of mechanical strains. P values were adjusted using Bonferroni correction for multiple comparisons in a–d, f, and g.
